Good thing takes time

Reference 1:
Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5695-9.
Cheng S,Fockler C,Barnes WM,Higuchi R .
Department of Human Genetics, Roche Molecular Systems, Inc., Alameda, CA 94501

Reaction : 8% Glycerol, 2%DMSO, 0.2mM dNTP, 0.5 uM primer set, Taq 5u/pfu 0.2u mixture.

Cycle (2 steps PCR):
94oc 10 sec preheat.
94oc 10 sec (denaturation)
Tm 5-22 min (annealing and extension)


Reference 2:
Nucleic Acids Res. 1992 Sep 11;20(17):4567-73
Extension of base mispairs by Taq DNA polymerase: implications for single nucleotide discrimination in PCR
Huang MM,Arnheim N,Goodman MF
Department of Biological Sciences, University of Southern California, Los Angeles 90089-1340