Good thing takes time

Recent advances in the TRAP assay:

New Cx reverse primers by Kim & Wu (Nucleic Acids Research 25:3595-2597, 1997) reduce primer dimer problems:

ACT primer: GCGCGG[CTAAACC]3

ACX primer: GCGCGG[CTTACC]3CTAACC

Redesigned primers by Miura et al.(Biochem. Biophys. Res. Comm. 246:13-19, 1998) may be more sensitive and semi-quantitative:

(first 25 cycles) forward primer: ATTGCCAATCCGTCGAGCAGAGTT

(last 2 cycles) forward primer: 32P-GCCAATCCGTCGAGCAGAGTTAGGG

(all cycles) reverse primer: CTCCC[TAACCC]3

website:

http://images.google.com/imgres?imgurl=http://www.genlink.wustl.edu/teldb/info/image/trap.gif&imgrefurl=http://www.genlink.wustl.edu/teldb/info/greider.html&h=490&w=473&sz=9&hl=en&start=17&um=1&tbnid=jkPH38Bf7whlyM:&tbnh=130&tbnw=125&prev=/images%3Fq%3DTRAP%2B%2Bassay%2Bprotocol%26svnum%3D10%26um%3D1%26hl%3Den%26client%3Dfirefox-a%26rls%3Dcom.google:en-US:official%26sa%3DN