Good thing takes time

Proteomics
1. ionsource:

2. ion fragmentation calculator:

3. Rockefeller Chait lab:

Organic synthesis:

1. organic-chemistry- you can find almost every name reaction (like Suzuki coupling)
http://www.organic-chemistry.org/

2. Synthetic page
http://www.syntheticpages.org/

3. Journal and experiment resourse
http://chem.wayne.edu/andreanagroup/link1.htm

4. pka database at UW (Bordwell pKa table)
http://www.chem.wisc.edu/areas/reich/pkatable/

5. NMR introduction by Joseph P. Hornak, Ph.D.

6. Chemiluminescence
http://www.chm.bris.ac.uk/webprojects2002/fleming/index.htm

7. Chemexper.com - it gives you the IR stuctures of the compound:
http://www.chemexper.com/


Molecular biology:

1. IDT - you just put a sequence here, it can find many pairs of primer for you .
http://scitools.idtdna.com/biotools/primer_quest/primer_quest.asp#

2. Baylor college of medicine - want to know the complement sequence of your DNA?
http://searchlauncher.bcm.tmc.edu/seq-util/Options/revcomp.html

Clinical Information:

1. NCCN (National Comprehensive Cancer Network)

Database Resource:

1. DTP:( Developmental Therapeutics Program NCI/NIH)
Chemicals and 60 tumor cell line information

2.Center of Cancer Experimental Therapeutics/ KU
for HTP screening project

3. CRISP
for grant searching

4. Funding Opportunities (RFAs, PAs, SBIR, STTR)

5. Grant.gov

MTV:

My collection:

Friends manuscripts:

Times Magazine: 23221536730 Yahoo Dictionary: Dictionary.Com

Myself:

Step 1: Preconjugation of ATM antibody
antibody( Novus: NB100-104) 2.5 ug
Protein A - sepharose slury 30 uL
TBST 200 uL
in 0.5mL tube

o/n 4 degree.

Step 2: reaction( depend on your purpose)

Step 3: ATM IP by Protein A-ATM antibody complex bead
reaction (about 100-50 uL) + complex
4 degree 2 hours.
wash.

Step4: Kinase reaction

ATM IP complex + P53-GST fusion peptide(10 ug)
buffer C: 10uL
150 mM KCL: 10 uL
GammaP(32) ATP: 1 uL

32 degree for 30 min.

Step 5: GST poul-down

Glutathione bead: 30 uL
4 degree, 2 hours

Step 6: 60 uL SDS lameli buffer boiling

Tricks: using long tip to suck out all the buffer in the beads.

wash by TBST 1-2 times


A Luminol/iodophenol chemiluminescent detection system for western immunoblots